Comparison of 4', 6'-diamidino-2-phenylindole and Giemsa Stainings in Preimplantation Mouse Embryos Micronucleus Assay Including a Triple Dose Study
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Comparison of 4', 6'-diamidino-2-phenylindole and Giemsa stainings in preimplantation mouse embryos micronucleus assay including a triple dose study.
Analysis of micronuclei (MN) in preimplantation embryos is a good method for the evaluation of cytogenetic damage induced by occupational and environmental mutagen during early pregnancy. To examine whether conventional Giemsa staining produced the same accuracy of micronuclei as the DNA-specific 4', 6'-diamidino-2-phenylindole (DAPI) staining in preimplantation embryo induced by maternal expos...
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Two-dimensional NMR spectroscopy has been applied to study the solution binding of 4',6-diamidino-2-phenylindole (DAPI) to synthetic DNA duplex [d(GCGATCGC)]2. The structure of the complex at a molar ratio of 1:1 drug:duplex has been investigated. NMR results indicate that DAPI binds selectively in the minor groove of the DNA region containing only two A:T base pairs. The results disagree with ...
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ژورنال
عنوان ژورنال: INDUSTRIAL HEALTH
سال: 2007
ISSN: 0019-8366,1880-8026
DOI: 10.2486/indhealth.45.343